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goat anti human igg secondary antibody conjugated with hrp  (SouthernBiotech)


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    SouthernBiotech goat anti human igg secondary antibody conjugated with hrp
    Goat Anti Human Igg Secondary Antibody Conjugated With Hrp, supplied by SouthernBiotech, used in various techniques. Bioz Stars score: 95/100, based on 110 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/anti+human+fc+secondary+antibody/Goat+Anti-Human+IgG+Fc%2C+Multi-Species+SP+ads-HRP/pm41820555-388-10-18
    Average 95 stars, based on 110 article reviews
    goat anti human igg secondary antibody conjugated with hrp - by Bioz Stars, 2026-09
    95/100 stars

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    Incubation:

    Article Title: Anti-CD47 x anti-mesothelin antibodies and methods of use thereof
    Article Snippet: .. In brief, increasing concentrations of MSLN Mabs and biAbs were incubated with CHO-huMSLN cells or CHO-cyMSLN cells for 30 minutes at 4° C. After two washes, bound antibody was detected using PE-conjugated anti-human Fc secondary antibody (Southern Biotech #9042-09). .. In brief, increasing concentrations of MSLN Mabs and biAbs were incubated with CHO-huMSLN cells or CHO-cyMSLN cells for 30 minutes at 4° C. After two washes, bound antibody was detected using PE-conjugated anti-human Fc secondary antibody (Southern Biotech #9042-09).



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    A . The binding of anti-gp41 7B2 to H9/N:L4-3 cells was demonstrated by indirect immunofluorescence and flow cytometry. The gray histogram indicates binding in the presence of secondary FITC-conjugated goat anti-human IgG only, the blue histogram demonstrates fluorescence of cells first incubated with 7B2 followed by FITC-conjugated secondary antibody. B. Comparative cytotoxicity of 7B2-dgA and 7B2-PNU. Cells were incubated with the indicated concentration of CIC and CD4-IgG2 (500 ng/mL) for 72 hr. MTS dye reduction was measured during the final 3 hr of culture. C. The kinetics of apoptosis induction by the CICs was measured by the binding of fluorescent annexin V to cells at different times following treatment with CIC.

    Journal: bioRxiv

    Article Title: Anti-HIV Immunotoxin and Antibody-Drug Conjugate Display Both Common and Distinct Effects in Killing Target Cells

    doi: 10.64898/2026.04.07.717054

    Figure Lengend Snippet: A . The binding of anti-gp41 7B2 to H9/N:L4-3 cells was demonstrated by indirect immunofluorescence and flow cytometry. The gray histogram indicates binding in the presence of secondary FITC-conjugated goat anti-human IgG only, the blue histogram demonstrates fluorescence of cells first incubated with 7B2 followed by FITC-conjugated secondary antibody. B. Comparative cytotoxicity of 7B2-dgA and 7B2-PNU. Cells were incubated with the indicated concentration of CIC and CD4-IgG2 (500 ng/mL) for 72 hr. MTS dye reduction was measured during the final 3 hr of culture. C. The kinetics of apoptosis induction by the CICs was measured by the binding of fluorescent annexin V to cells at different times following treatment with CIC.

    Article Snippet: Cells were washed twice in PBS and incubated with 2 μg /mL of secondary FITC-conjugated goat anti-human IgG (Novus Biologicals, Centennial CO USA) for 1 hr at room temperature in the dark, then washed a final time with PBS and resuspended in 2% paraformaldehyde.

    Techniques: Binding Assay, Immunofluorescence, Flow Cytometry, Fluorescence, Incubation, Concentration Assay